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For example, studies involving the cryopreservation of ram semen have revealed that freezing leads to the opening of the mitochondrial permeability transition pore (mPTP), resulting in increased permeability of the inner mitochondrial membrane, which leads to an elevation in calcium (Ca2+) and ROS release ( in vitro fertilization and artificial insemination ( 4.4 Antioxidant therapy Antioxidant therapy is considered one of the crucial strategies for improving sperm quality ( 5 Conclusion To sum up, this study is the first to systematically analyze the studies on oxidative stress and male fertility in the past 10 years using bibliometrics, and successfully establish a knowledge map in this field