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Description
SDS-PAGE and SYPRO Ruby staining Due to the high burden of SDS in our protein extracts, protein concentrations in trichome and leaf extracts were determined by the detergent-resistant Pierce BCA protein assay (Thermo Fisher Scientific, Waltham, USA)
Both suppress the signal transducer and activator of transcription 3 (STAT3) signaling pathway, reducing proangiogenic gene expression and VEGF-A release
Watanabe M, Houten SM, Wang L, Moschetta A, Mangelsdorf DJ, Heyman RA, et al
Improves metabolic function, fat-burning efficiency, and weight balance
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Methods used to elute the protein of interest include changing the pH, or adding a competitive molecule, such as imidazole
