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doi: 10.1161/JAHA.115.003046

Small molecule inhibition of cGAS reduces interferon expression in primary macrophages from autoimmune mice

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Western blotting For whole-cell protein extraction, cell pellets were lysed in cold RIPA buffer (50 mM TrisHCL pH 7.4, 5 mM EDTA, 250 mM NaCl, 50 mM NaF, 0.1 mM Na 3 VO 4 , 0.1% Triton X-100), supplemented before use with cOmplete Protease Inhibitor Cocktail (Roche) and 5 mM sodium butyrate

Additionally, ACTH stimulation may improve the sensitivity of PACs as a clinical indicator of nocturnal hypertension