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Total RNA was isolated from the HK-2 cells using ISOGEN (Nippon Gene Co., Ltd.)
The breakdown of casein by chymosin or milk-based enzymes such plasmin and cathepsin D is known as primary proteolysis
Protein lysates were then cleared in a refrigerated table-top microcentrifuge (Eppendorf) at 21,100 g for 10 min
Martinez et al., 2017), and induces strong Th1 immune responses and antibodies against -glucans and mannans (Liu et al., 2011)
