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While marketed as an anti-aging therapy, more robust studies are needed to validate these claims

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Supplementation with GSH is generally ineffective at increasing cellular glutathione levels because most human cells cannot uptake extracellular GSH due to the lack of the membrane-bound ectoenzyme -glutamyltranspeptidase (-GT) or due to an unfavorable concentration gradient across the cell membrane 10,16

The position of GSH in the model is supported by data showing that mutation of two of the arginines, R73 and R74, results in complete loss of substrate saturation with GSH (with CDNB as the electrophilic second substrate)