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In vitro, Hypo-Exos suppressed LPS-induced proinflammatory cytokine release and reduced ROS in activated microglia, consistent with their role in promoting M2 macrophage polarization [42]

Drucker DJ, Nauck MA

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Direct water injection onto powder Squirting water directly onto the lyophilized powder creates impact force that damages peptide structure

For the alkaline washed samples, the additional alkaline wash was performed with the 100 mM sodium carbonate, pH 11.0 solution for 6 CV and the subsequent neutralisation with PBS pH 7.2 for 8 CV

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