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Visualization and quantitative assessment of the brain distribution of insulin through nose-to-brain delivery based on the cell-penetrating peptide noncovalent strategy

The uptake of Cys into neurons is mainly mediated by excitatory amino acid carrier 1 (EAAC1, in rodents), also known as excitatory amino acid transporter type 3 (EAAT3, in humans) (Figure 3)

Each reaction included 8 L of diluted cDNA, 10 L of Kapa SYBR FAST qPCR kit reagents and 1 l each of 5 M stocks of the forward and reverse primers in a total reaction volume of 20 L