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doi: 10.1046/j.1440-1746.2003.03135.x

PA14 from overnight cultures was spread on either high osmolarity PGS (pepton-glucose-sorbitol) agar plates for fast killing assays or 140% Bacto-peptone NGM agar plates for mRNA expression assays were prepared according to ref

1C), suggesting that the role of acdh-11 homologs in the sequestration of -cyclopropyl fatty acids may also be conserved

Diabetic keratopathy: redox signaling pathways and therapeutic prospects

The RNA was eluted in 60 l of elution buffer

Notably, we observed the delipidation of VIT-2::GFP-containing particles in embryos with NR 2p-FLIM (separate GFP and Nile Red signals in the embryos shown in Supplementary Figure S5), in agreement with the previous BCARS finding ( Under low-concentration Nile Red vital staining conditions, most of the Nile Red molecules have been reported to label acidic gut granules rather than lipid particles in the intestine (Supplementary Figure S3)