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The heterogeneity of the uterine microbiota is likely shaped by a combination of host and environmental factors, including genetic background, hormonal fluctuations, immune characteristics, and sexual intercourse 88,89,90

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FIGURE 1 In the first set of experiments, animals were separated into one of the following groups ( n = 6 per group): Control (0.9% saline followed by 0.9% saline), C/Control (caffeine followed by 0.9% saline), C/L-theanine (caffeine followed by L-theanine), C/Mg-T1 [caffeine followed by Magnesium-L-theanine (8% Mg 2+ )], or C/Mg-T2 [caffeine followed by Magnesium-L-theanine (18% Mg 2+ )]

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doi: 10.3389/fnut.2025.1679494 Received 04 August 2025 Revised 19 October 2025 Accepted 25 December 2025 Published 22 January 2026 Volume 12 - 2025 Edited by Manuela Machado, Escola Superior de Biotecnologia - Universidade Catlica Portuguesa, Portugal Reviewed by Ivan oa, University of Rijeka, Croatia Moumita Saha, Yale University, United States Updates Copyright 2026 Chang, Lin, Renn, Liu, Yen, Liao, Tikhonova, Amstislavskaya, Singh and Chen

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