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The expression of these markers thus confirmed the successful differentiation of cerebral organoids in vitro

[DOI] [PMC free article] [PubMed] [Google Scholar] 242

Cell viability was determined by absorbance, at 595 nm wavelength, with a microplate reader in a SpectraMax M2 (Molecular Devices, Sunnyvale CA)

Tumor necrosis factor (TNF)- expression was determined with a LightCycler primer and probe set (Nihon Gene Research Laboratories, Sendai, Japan)
[DOI] [PubMed] [Google Scholar] 20.Gollnick HP, Finlay AY, Shear N
