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Cell transfection The endotoxin-free plasmids were prepared with plasmid miniprep plus purification kit (DP01-Plus-300, GeneMark), purified by ethanol precipitation, and subjected to cell transfection

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--- COMPARISON Bacteriostatic Water vs Sterile Water: Research Solvent Comparison Bacteriostatic Water Understanding the difference between bacteriostatic water vs sterile water is essential for proper peptide reconstitution in research settings

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This preparation consists of Water for Injection, USP preserved with 0.9% (9 mg/mL) benzyl alcohol, which functions as a bacteriostatic antimicrobial agent inhibiting microbial growth in opened containers during laboratory use

doi: 10.1016/j.foodchem.2023.137652 202