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To ensure the absorbance from oil red O staining was within the linear detection range of the plate reader, a calibration curve was established for oil red O staining in adipocytes using a previously described protocol.[23] 2.12

References 1 AksoyS.SzumlanskiC

In that case, the patient should skip the missed one and continue on the regular schedule

Results may vary

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Its selectivity is just as important