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After de-paraffinization and rehydration, sections underwent a heat-induced epitope retrieval by microwave heating in 0.1 M TRIS hydrochloride (HCl) (pH 9.0) for 15 min

The epigenetic regulatory roles of curcumin include the inhibition of DNA methyltransferases (DNMTs), regulation of histone modifications via the regulation of histone acetyltransferases (HATs) and histone deacetylases (HDACs), regulation of microRNAs (miRNA), action as a DNA binding agent and interaction with transcription factors

Additionally, cfDNA and oxLDL contribute to atherosclerosis by activating TLRs and NLRP3 inflammasomes, regulating downstream signaling pathways, and inducing macrophage differentiation into foam cells [306, 311]

( John Wiley & Sons, Ltd), 124

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