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To further explore the impact of inhibiting ALA synthesis on small intestine in vivo, young mice were injected with 50 mg/kg elesclomol, while young and old mice injected with vehicle served as controls (as depicted in Fig

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Samples were analysed using a flow cytometry analyser (FACS LSR II SORP or Fortessa X20, BD Biosciences) at a rate of less than 7,500 events per second

We thus made use of TPP, which measures thermal stability by monitoring protein aggregation
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