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The coding region was cloned into a modified pETM-11 bacterial expression vector which was derived from a pET-24d(+) vector (Novagen) by insertion of a tobacco etch virus (TEV) protease cleavage site following an N terminal hexa-histidine and protein A tag

We transfected AC16 cells with reporter plasmids containing PPARGC1A promoter deletion mutants, pGL3BV- PPARGC1A -1631, pGL3BV- PPARGC1A -1386, pGL3BV- PPARGC1A -1012, and pGL3BV- PPARGC1A -210, and treated them with 50 M rosiglitazone, 50 M WY-14643, or a combination of both

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