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The goal is a clear, stable solution at a known concentration (e.g., 1 mg/mL or 2 mg/mL) that allows accurate pipetting or syringe draws

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Controls received either saline (5 mL/kg) intraperitoneally or drinking water (12 mL/rat/day) till the sacrifice, at 7, 14, 28, and 42 postoperative days