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Subsequently, the mixture was diluted five times with a buffer containing 20 mM MES, pH 6.0, 100 mM LiCl, and then loaded to HiTrap QFF anion exchange column for further purification

Notably, NETs not only possess antibacterial functions but also can entrap host cells and release damage-associated molecular patterns (DAMPs), thereby further amplifying inflammatory responses and thrombosis, which intensifies tissue damage and microcirculatory dysfunction

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These treatments cannot be used over long periods of time due to severe adverse effects such as depression and bone loss

A., Smith, S

To analyze the endogenous complex formation in vivo between MAGE-G1 and VIME during neuronal differentiation, we carried out endogenous immunoprecipitation in P19 cells after 6-day treatment with RA