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Primary antibodies used were as follows Ki67 (Abcam, ab16667, 1:200), CEA (Huabio, ET1705-52, 1:500), -SMA (STARTER, S0B2103, 1:200), Vimentin (Huabio, ET1610-39, 1:500), Nucleolin (Abcam, ab129200, 1:200), and CTNNB1 (Proteintech, 51067-2-AP,1:200)

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