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The treated mice showed several other improvements

Depletion of serum holotranscobalamin

Without ongoing B12 replacement, deficiency and symptoms will typically recur within months to years of discontinuation

Medications that commonly promote weight gain include certain antipsychotics, antidepressants (particularly mirtazapine and paroxetine), corticosteroids, insulin, sulfonylureas, some beta-blockers, and anticonvulsants like gabapentin

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This approach isolates each compound's contribution and ensures any adverse interactions are detected before full-dose stacking