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They are not medicines and are not intended to treat, diagnose, mitigate, prevent, or cure diseases [124]

Without sufficient antioxidant defenses, toxins and oxidative stress can accumulate

It leads to the formation of Au@GSH nanoparticles, meaning that the surface of Au NPs is capped with a molecular layer of GSH and expose with carboxylic acid groups

The extracellular thiol/disulfide red-ox environment (determined by the interconversion between cysteine and cystine) is able to modulate cell proliferation, apoptosis, cell adhesion molecules, and proinflammatory signaling

For intracellular staining of hexokinase-1, cells were stained for extracellular markers, fixed in 4% FA at RT, and stained with Anti-Hexokinase 1 (Abcam ab184818) diluted 1:100 in PBS 0.1% Tween-20 at RT