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Nuclei were then stained with DAPI solution in PBS at 37 C for 10 min in the dark and washed three times with PBS

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doi: 10.1038/srep33407 311 BordignonPBottoniGXuXPopescuASTruanZGuenovaEet al

The reaction was monitored at an absorbance of 600 nm for 2 min with a BioTek Epoch 2 spectrophotometer

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