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For sequential image acquisition, the following imaging parameters were used: excitation 405 nm (emission 450/50 nm) for Hoechst and excitation 561 nm (emission 600/50 nm) for Alexa Fluor 568 with 50 ms and 200 ms exposure time, respectively

We observed co-degradation events

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Upon chronic stimulation by pathologic insults, microglia become persistently activated and release pro-inflammatory molecules like tumor necrosis factor- and interleukin-1, promoting neuroinflammation and neuronal damage (Leng and Edison, 2021

These changes are the result of the stimulation of the transcription of the gene responsible for melanin production