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Protein extraction and western blot analysis Cultured cells were lysed in radioimmunoprecipitation assay lysis buffer (Beyotime, Shanghai, China) supplemented with phenylmethylsulphonyl fluoride and protease inhibitor cocktail (Sigma-Aldrich, USA) on ice for 10 min and centrifuged at 12,000 g for 10 min at 4 C

Formulated to target visible signs of aging and sun damage, this product blends ingredients that support skin firmness and elasticity

McPherson et al., 2012)

Pathogen-free Ixodes scapularis larvae were purchased from Oklahoma State University Tick Rearing Facility (Stillwater, Oklahoma, USA)

The hepatocytes were then dissociated by the digestion buffer containing 0.2 g/L collagenase IV and purified by 50% Percoll density separation

The protocols shift depending on what youre trying to accomplish, so heres how Id break it down by specific goal