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Scoring fitness and vitamin B 6 remediability Each sequencing experiment contained not only libraries derived from selective and non-selective pools, but also libraries derived from wildtype amplicons, enabling estimation of the component of each mutations observed allele frequency in the pool that was derived from PCR errors during library preparation or sequencing errors

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After incubation with collagenase IV, cells were separated by mechanical destruction of tissues, filtered through a 100 m cell filter, and then, centrifuged at 700 rpm for 2 minutes to obtain cell precipitates

This process involves the intravenous administration of chelating agents like calcium-EDTA and DMPS, which bind to these harmful metals and facilitate their elimination through the kidneys