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Nutritional status: Fasting and protein malnutrition reduce hepatic NAD+ availability, impairing ADH pathway efficiency and slowing the rate of ethanol oxidation per hour

List of abbreviations: COPD: chronic obstructive pulmonary disease

Funding Statement This research was funded by a JSPS KAKENHI Grant-in-Aid for Scientific Research (C) (21K06850) to J.F

GSH depletion causes ferroptosis in RPE cells To evaluate the mechanism of cell death in GSH-depleted cells, we treated RPE cells with various cell death pathway inhibitors including ferroptosis inhibitors ferrostatin-1 (Fer-1, 8 M), liproxstatin-1 (Lip-1, 600 nM), iron chelator deferoxamine (DFO, 80 M), pan-caspase inhibitor z-VAD-fmk (30 M) to inhibit apoptosis, autophagy inhibitor 3-methyladenine (3-MA, 10 mM), and lysosomal inhibitor bafilomycin A1 (Baf-A1, 75 nM)

The pre-mRNA in the constructs was engineered to splice and produce ihpRNAs with short loops

This clear distinction is indicated on the labels of research chemicals, exempting them from specific regulations under Title 21, Parts 100-740 of the Code of Federal Regulations (21CFR)