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After 5 min at 95C followed by centrifugation for 10 min at 12,000 g, 20 L of the resulting supernatant was diluted with 80 L deionized water for subsequent aldehyde determination

The hemoglobin proteins packed into red blood cells are an example (see Figure \(\PageIndex{9 \))

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NADH donates an electron at Complex I, generating NAD + , while FADH 2 donates two electrons at Complex II, generating FAD

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