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study highlighted the dysregulation of glutathione metabolism in septic patients and emphasized that the impaired biosynthetic response may contribute to heightened oxidative damage and worsened clinical outcomes (Liu et al., 2024)

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2.8 Western blotting Proteins were extracted from the tissue samples, C2C12 myocytes, or HUVECs using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Nacalai Tesque, Kyoto, Japan), separated using SDS-PAGE, and transferred to PVDF membranes

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In this method, glutathione is enclosed inside protective lipid layers

Black cumin (Nigella sativa) and its constituent (thymoquinone): a review on antimicrobial effects
