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The proteins in the pulldown were resuspended in sample buffer, samples were resolved by NuPAGE gel electrophoresis and anti-MycMunc13-4 (71D10, Cell signaling) or anti-FlagMunc13-4 (Genecopoeia, CGAB-DDK-0050) and GST (Sigma, 06-332) were detected by western blot

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These findings highlight the importance of ketones in neural development and suggest that a range of sensory neuron lineages are affected by the absence of ketolysis in sensory neuron mitochondria

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doi: 10.1155/2013/760804 49

A more recent methodology, the CIPN-R-ODS, was developed with Rasch analysis to build overall disability scales that provide a linear measurement of CIPN-related disability and will likely be utilized in future CIPN clinical trials 22