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For negative controls, the staining solution included 5 mM serine (Sigma-Aldrich, S4311) and 10 mM sodium borate (Sigma-Aldrich, 71997)
Bger, R
10.1016/j.immuni.2015.02.002 339 WenY.-D.ZhuY.-Z
Invest New Drugs (2012) 30:230317
Finally, the autophagosome fuses with lysosomes to degrade their content or fuses with the plasma membrane for exocytosis
The method of 1 O 2 capture is extremely efficient and it has been estimated that 1 -tocopherol molecule can neutralize up to 220 molecules of 1 O 2 in vitro before its degradation (Sharma et al., 2012)
