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The field of proteomics has benefitted from rapid increases in analytical and computational capabilities since the field was initiated in 1992 and, at the time, relied primarily on large 2D gels to separate the proteins

further demonstrated visual biomarkers of iron deposition within the pulvinar region of the thalamus in epilepsy patients using SWI (72)

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The secondary indicators apply to patients with mild or moderate alopecia and suggest upgrading the severity level if one or more of the following conditions are present: the alopecia significantly affects the patient's psychological or social wellbeing, there is substantial involvement of eyebrows or eyelashes, there is no notable improvement after at least 6 months of treatment, or the hair pull test is positive, indicating rapid disease progression