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Description
Effect of antioxidants on cultured human diploid fibroblasts exposed to cystine-free medium

Lysates from cells that were harvested at the indicated time points were analyzed by WB

Protein silver-staining assay Anti-Flag co-immunoprecipitation (Co-IP) products from HEK 293 T cells transfected with Flag-SLC25A39 were separated by SDS-PAGE and subsequently visualized using a Silver Staining Kit (CWBIO, CW2012)

J.LiuN.LiuJ

Many users in these forums attribute this reaction to acute mast cell activation triggered by the peptide complex, noting that they require the concurrent, daily use of potent systemic antihistamines (such as Xyzal) simply to tolerate the basic administration of the protocol

doi: 10.1021/ja908215t
