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Acquisition of 1 H-NMR spectra and metabolic profiling The lyophilized metabolic extracts were suspended in 0.6 ml of deuterated water (Cambridge Isotope Laboratories Inc., Tewksbury, MA, USA) containing phosphate buffer (100 mM) and TSP (3-3-(trimethylsilyl)-2,2,3,3-tetradeuteropropionic acid or TMSP- d 4 , Cambridge Isotope Laboratories) (0.5 mM) and inserted into a 5 mm NMR tube for immediate acquisition

doi: 10.1038/nm.2353 161 DeFuriaJBelkinaACJagannathan-BogdanMSnyder-CappioneJCarrJDNersesovaYRet al

Prevalence of Chronic Pain and High-Impact Chronic Pain Among Adults - United States, 2016

L., Riddle, M

Polymorphism of GSTs While polymorphisms have been identified within each class of GSTs, only the heterogeneity that may contribute to variations in pathology or clinical response will be addressed in this review

GST (Glutathione S-transferase) can specifically bind to glutathione, demonstrating the principle of enzyme and substrate interaction