gst-glutathione affinity purification Transglutaminase 2 crosslinks the glutathione S-transferase tag, impeding protein–protein interactions of the fused protein Chromatography (Part 2 of 2):

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DAG helps activate protein kinase C (PKC), which phosphorylates many other proteins, changing their catalytic activities, leading to cellular responses

doi: 10.1002/iub.1068

In: Exploration of Host Genetic Factors associated with Malaria

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The staining solution, containing 5 ml DMEM, 8 l FDA (5 mg/ml) and 50 l PI (2 mg/ml) was added to the wells, cells were incubated at room temperature for 4-5 minutes in the dark, washed with PBS and examined on Eclipse Ti-E confocal laser scanning microscope (CLSM) with A1 confocal module (Nikon Corporation, Japan) and CFI Plan Apo VC 20/0.75 objective lens with a life support system, maintaining normal culturing conditions (37C, 5% CO 2 )