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Along with the appearance of a considerable number of endothelial lesions following ligation, an initial blood flow deprivation and a presumably inadequate response, decreased NO levels (Figure 7) and increased MDA levels (Figure 8) were observed in the colon tissue

The cells were washed twice with 100 mM potassium phosphate buffer (pH 7.0) containing 0.1 mM PMSF

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Western blotting analysis was performed as previously described [8]