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We preferred to use pH 7.2 to further reduce non enzymic hydrolysis without affecting the linear relationships of the assay procedure at 37C.Moreover, the effect of temperature on the pH of the phosphate buffer is small therefore the buffer can be prepared at room temperature and used at a temperature ranging 1040

37 Secondly, biologics prescribed for IBD can also be prescribed for other immuno-inflammation-related diseases such as rheumatoid arthritis, psoriasis and psoriatic arthritis, which were not excluded in the present analysis, therefore such comorbid conditions may also have contributed to the overall results

Bush NG, Diez-Santos I, Abbott LR, Maxwell A

The authors also examined the phototoxicity of curcumin in mammalian cells (rat basophilic leukemia cells) and demonstrated that the death profile of this cancer followed a first order kinetic [89]

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