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The recycling reaction was initiated by the addition of reaction buffer (100 mM sodium phosphate, pH 7.4), supplemented with 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) (100 M) (Sigma), NADPH (100 M) (Roth), glutathione reductase (1 U/ml) (Sigma), and EDTA (1 mM) (Sigma)

Figure 8 illustrates that the genes GSK3B and APOE are functionally connected to ITM2B through the APP gene, which plays a crucial role in regulating the processing of the amyloid precursor protein

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BPC-157 is used as an adjunct, not a replacement, for standard post-op care