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Future medicinal chemistry efforts may yield next-generation compounds with enhanced properties, including improved tissue selectivity, modified pharmacokinetics, or additional mechanistic activities that complement NNMT inhibition

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Glutathione agarose beads are cleaned and regenerated by alternating several column volumes of: 1) 0.1M Tris-HCl pH 8.5, 0.5M NaCl, and 2) 0.1M sodium acetate pH 4.5, 0.5M NaCl