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Harnessing amino acid pathways to influence myeloid cell function in tumor immunity

Growth hormone and IGF-1 increase bone mineral content and density by promoting osteoblast activity while reducing osteoclast-mediated bone resorption

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ATF4 activation attenuates the mitophagy process despite increase in mitophagy-initiator proteins under chronic ER stress, thereby contributing to CEnC viability To determine whether mitophagy initiators are affected in the mitochondrial fraction under ATF4 manipulation after chronic ER stress, we knock down ATF4, induce chronic ER stress using Tun, isolate mitochondria from 21T cells, and probe for Parkin, PINK1, and LC3II