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The important one is that affinity chromatography involves many types of interactions between ligand and target such as steric effects, hydrogen bonding, ionic interactions, van der Waals forces, dipol-dipol interactions and even covalent bonds while other chromatographic techniques involve just one or a few of them

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Guo J, Ping Y, Ejima H, Alt K, Meissner M, Richardson JJ, et al

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The upper acetonitrile layer was transferred by glass pipette to a 2 mL dSPE tube containing 100 mg of PSA (UCT, Bristol, PA)