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Insular cortex neurons encode and retrieve specific immune responses

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Cleaved protein was repurified from the cleaved fusion tag and TEV protease by loading on a Ni-NTA resin column and 5 ml of the flow through was applied to a size-exclusion column (HiLoad Sepharose 16/600 75 pg, GE Healthcare) on an KTA Pure system (GE Healthcare) at RT

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Accordingly, decreased cAMP levels were observed in fly and mouse FXS models and a deregulation of cAMP and cGMP was also identified as a molecular hallmark in FXS patients (99, 100)