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Rats receiving a high oral dose of 500 mg/kg body weight showed plasma and brain EGCG concentrations of 12.3 nmol/mL and 0.5 nmol/g, respectively [31]

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However, ingesting prebiotics does not directly impose these effects

The accumulation of active species such as ROS will cause lipid peroxidation, and the accumulation of its products and reactive degradation products will result in ferroptosis

Samples were heated at 95 C for 10 min then cooled off at room temperature

NAD+ deficiency may also result in the methylation of cellular DNA so that genes are not expressed appropriately