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Uniform gain and exposure settings in the Nikon NIS Imaging Software with identical objective lens magnification of 40 with a numerical aperture of 0.75 were applied to all sections to ensure consistency in image intensities and magnification

[DOI] [PMC free article] [PubMed] [Google Scholar] 85.Frisoli M.L., Harris J.E

The cell presents fragments of the spike protein on its surface

Disaggregation Protocols: For peptides with severe aggregation tendency, specialized disaggregation protocols using TFA/HFIP treatment may be necessary before final reconstitution in aqueous buffers

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Dit anion splitst dan snel een disulfide binding (Cys 58 - Cys 63 ) waarbij Cys 63 vrij komt, dat vervolgens als nucleofiel reageert met de dichtstbijzijnde sulfide eenheid in het GSSG molecule (bevorderd door His 467 ) tot een gemengde disulfide binding (GS-Cys 58 ) en een GS anion