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Could it possibly be the B vitamins

The percentage of cytotoxicity for each of the assigned agents was calculated according to the formula: where OD control = optical density of the untreated cells, and OD sample = optical density of the treated cells

However, a surprisingly low in vivo efficacy was observed, particularly on day 2 after administration, indicating that these compounds with pI in the basic range probably have a short biological half-life in rats

Medicine 103 , e37360 (2024)

Simply stated, during times of biological stress (inflammation, illness, infections, etc.) or real, even perceived danger (psycho-emotional stress, loss, overworking, etc.), or a combination of both, the stress response turns down or blocks chemicals in the brain and body that under normal circumstances promote physical and mental relaxation, positive mood, sleep, et cetera

The primer sequences listed in Supplementary Material 1 Table S1 were designed to span exon-exon junctions to avoid amplification of genomic DNA