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After overnight incubation, the medium was changed to 90% of Williams E Medium and supplemented with 12.5% HepaRG preinduction and toxicity medium supplement (Lonza, catalog: MHPIT) and 25 U/mL penicillin-25 g/mL streptomycin, followed by 72 h incubation

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doi: 10.1371/journal.pone.0025792

Adding BPC-157 and TB-500 to either protocol extends the training tolerance that determines how much of the hormonal signal actually converts to muscle tissue