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This was followed by vortex mixing for ~1 min before centrifuging samples at 10,000 relative centrifugal force (RCF) for 20 min at 4C

TMAU can present in two forms: Primary TMAU: A rare genetic disorder caused by mutations in the FMO3 gene, resulting in a congenital deficiency of the enzyme [2]

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And the mixability was great
