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Add 100 mL/well (96-well plate) of Master Reaction Mix into the cell plate

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For detection, ImmPRESS HRP Horse Anti-Goat IgG Polymer Detection Kit (Vector Laboratories, Catalogue# MP7405) was used

It activates phase 2 enzymes by signaling through nuclear factor erythroid 2related factor 2 (Nrf-2) and interacting with the Keap1 protein

The tremor decreases when the hand is moving purposefully, may be worsened by fatigue or stress, and disappears completely during sleep

The most striking example of this is the dramatic difference seen between the clinical stages of tumours with a Gleason score of 7, depending on whether there was a primary pattern of 3 and a secondary pattern of 4, and vice versa (3 + 4 = 7, and 4 + 3 = 7, respectively)