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Neuroimaging studies have confirmed that reduced gray matter volume in the dorsolateral prefrontal cortex (DLPFC) of long-term smokers directly correlates with a decline in executive function, whereas compromised cingulum bundle integrity affects emotional regulation [164, 165]

doi: 10.2147/JIR.S312783 137 CaoFHuangCChengJHeZ

Consequently, dihydrolipoyl transacetylase cannot accept the hydroxyethyl group from TPP because it is maintained in the acetylated form

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thus, GCL is considered the rate-limiting enzyme [6]