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Research demonstrates that copper-binding peptides including GHK-Cu suppress the NF-B pathway through direct molecular interactions, with nuclear translocation of NF-B p65 decreased by approximately 30% upon treatment and molecular modeling showing specific peptide sequences exhibit high binding affinity to NF-B p65 (up to -8.8 kcal/mol)

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For understanding the binding of the four selected compounds to GPX4 protein showing the mechanism of the interaction, molecular docking analysis and molecular dynamic (MD) simulation were used

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